Generator

Part:BBa_K812052:Design

Designed by: Cyrille Pauthenier   Group: iGEM12_Evry   (2012-09-20)


pLac promoter R0010 with GoldenBrick extension


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 17
    Illegal SpeI site found at 237
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 237
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 17
    Illegal SpeI site found at 237
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 17
    Illegal SpeI site found at 237
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7
    Illegal BsaI.rc site found at 231


Design Notes

The part R0010 has been amplified by PCR with the new GoldenBrick extension, and then cloned in pSB1C3 using EcoRI and PstI.


Source

This part has been created from R0010 by PCR amplification

References