Generator
Part:BBa_K812052:Design
Designed by: Cyrille Pauthenier Group: iGEM12_Evry (2012-09-20)
pLac promoter R0010 with GoldenBrick extension
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal XbaI site found at 17
Illegal SpeI site found at 237 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 237 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal XbaI site found at 17
Illegal SpeI site found at 237 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal XbaI site found at 17
Illegal SpeI site found at 237 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 7
Illegal BsaI.rc site found at 231
Design Notes
The part R0010 has been amplified by PCR with the new GoldenBrick extension, and then cloned in pSB1C3 using EcoRI and PstI.
Source
This part has been created from R0010 by PCR amplification